These were raised at Gen Con but I would like to have some clarification concerning the way quests work and I cannot find the answers in the rule book.
Can you have more than one active quest?
If you use an abiltity on a quest do you lose the resources?
I think the questions have straight forward answers...
1) There are no upper limit on the number of active quests mentioned in the rulebook, so I would say other than the resources and time that you are committing to them, no. Remember it takes some resource commitment and more than a few rounds to set up a quest in order to milk it, meaning it's a risky investment that may not pay off in the short (definately) and/or the long run (possibly). Since it's not a simple "point, click, and fire" mechanic, there are opportunity risks and costs involved that cannot be ignored. Haven't played yet, but I'm not sure taking significant opportunity and resources costs to get multiple quests going at any one time would be a staple strategy of mine.
2) From page 11 of rulebook: If the questing unit leaves play for any reason, the quest card remains in play but all resource tokens accumulated on that quest card are discarded, and returned to the pool in the centre of the table. So getting that one questing unit (remember you can only have 1 unit ever on a quest) killed due to card effects (its own as well as others) or during defense is a sure way to lose resources. Journey to the Gate (Chaos Quest) for example asks that you discard the questing unit to use its ability once you have 3 resources on it, meaning you have to build up the resource meter again by committing a new unit to it if you want to refresh it after activating the quest ability. Other than that it's up to the text on each individual quest card since there are no general rule that discards resources for using a quest ability.